ASYMMETRICAL FLOW FIELD-FLOW FRACTIONATION

Asymmetrical Flow Field-Flow Fractionation is the by far most advanced FFF technology for the characterization of proteins, polymers and nanoparticles in aqueous and organic solvents at ambient or high temperatures. Asymmetrical Flow Field-Flow Fractionation was first published by Giddings/Wahlund in 1987 [1]. Asymmetrical Flow Field-Flow Fractionation is a version of Flow Field-Flow Fractionation and differs by the way how the cross flow field is generated inside the Flow Field-Flow Fractionation channel. In Asymmetrical Flow Field-Flow Fractionation the cross flow is generated directly inside the channel. The cross flow is generated out of the main eluent flow which is pumped into the Asymmetrical Flow Field-Flow Fractionation channel. The flow is split and some part of the total flow is removed from the Asymmetrical Flow Field-Flow Fractionation channel via a semi-permeable membrane which is located in the bottom wall. The membrane pore size in Asymmetrical Flow Field-Flow Fractionation is selected in such a way, that only the solvent can pass through, but not the sample particles.

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[1] Wahlund, K.G.; Giddings, J.C.; “Properties of an asymmetrical flow field-flow fractionation channel having one permeable wall”, Anal. Chem., 59 1332-39 (1987).

Flow FFF  |  Asymmetrical Flow Field Flow Fractionation  |  Thermal FFF  |  Sedimentation FFF